Terpene Guide/Terpinolene
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C₁₀H₁₆ BP: 186°C (367°F) 0.05–1.0% in cannabis

Terpinolene

Complex, multifaceted aroma with sedating and antioxidant properties.

Terpinolene has one of the most complex aroma profiles of any cannabis terpene — simultaneously piney, floral, herbal, and citrusy. Despite its energetic scent, it tends to produce sedating effects. It is the dominant terpene in strains like Jack Herer and Dutch Treat and has demonstrated significant antioxidant, anticancer, and sedative properties in research.

Aroma Profile

FreshPineyFloralHerbalCitrus

Primary Effects

SedatingAntioxidantAnticancer (preclinical)Relaxing

Also Found In

Apples, Cumin, Lilac, Nutmeg

Mechanisms of Action

CNS Depression

Produces dose-dependent CNS depression in animal models, reducing locomotor activity and increasing sleep time via mechanisms not yet fully characterized.

Antioxidant Activity

Potent free radical scavenger. Reduces LDL oxidation and protects against oxidative stress-induced cellular damage.

Cell Cycle Arrest

Induces G1 phase cell cycle arrest in cancer cell lines, inhibiting proliferation without significant toxicity to normal cells.

Role in the Entourage Effect

Terpinolene is the dominant terpene in several classic sativa strains but produces paradoxically sedating effects. Its complex aroma contributes significantly to strain character and may modulate the overall effect profile through multiple receptor interactions.

Medical Applications

Insomnia

Moderate Evidence

Sedating effects documented in preclinical studies. Reduces locomotor activity and increases sleep time in rodent models.

Condition guide

Cancer (adjunct)

Preliminary Evidence

Inhibits cancer cell proliferation in multiple cell lines including brain, lung, and breast cancer. Mechanism involves cell cycle arrest.

Condition guide

Cardiovascular

Preliminary Evidence

Reduces LDL oxidation and demonstrates cardioprotective antioxidant effects in preclinical models.

Condition guide

Key Research

Preclinical2013Phytomedicine

Sedative effects of terpinolene in rodent models

Terpinolene produced significant dose-dependent sedation in mice, reducing locomotor activity by 45% at 100mg/kg, confirming CNS depressant activity.

In Vitro2020Oncology Letters

Terpinolene inhibits cancer cell proliferation

Terpinolene inhibited proliferation of brain, lung, and breast cancer cell lines with IC50 values of 15–45 μM via G1 cell cycle arrest.

In Vitro2022Food Chemistry

Antioxidant properties of terpinolene

Terpinolene demonstrated DPPH radical scavenging activity comparable to vitamin E and reduced LDL oxidation by 38% in ex vivo models.

Top Strains

Jack Herer

Sativa

0.5–1.5%

Dutch Treat

Hybrid

0.8–2.0%

Ghost Train Haze

Sativa

0.4–1.0%

XJ-13

Hybrid

0.3–0.8%

Chernobyl

Hybrid

0.4–1.2%

Safety & Considerations

GRAS status. Skin irritant at high concentrations. Safe at cannabis concentrations.

All terpene information is for educational purposes only. Consult a healthcare provider before using cannabis therapeutically.

Frequently Asked Questions